DNA content of presumed aneuploid spermatozoa

The DNA content of spermatozoa from different strains of mice.

Certain morphological characters of mammalian spermatozoa have been shown to have a high heritability (Beatty, 1970). Sperm dimensions may be altered by genetic selection (Woolley, 1970). In addition to the biological action ofthe genes, the actual mass of the DNA and proteins, which constitute the major components of the head, may affect the sperm phenotype. Unlike genetically active cells in ...

Analysis of chromatin integrity and DNA damage of buffalo spermatozoa

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

Techniques for augmentation of exogenous DNA uptake by ovine spermatozoa

Sperm mediated gene transfer can be an inexpensive and simple method in animal transgenesis; however its efficiency is poor, mainly due to the spermatozoa’s lesser uptake of exogenous DNA. In the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton X100 and DMSO, on exogenous DNA uptake by sheep spermatozoa and...

Origins of Dna Damage in Spermatozoa

Reproduction, Fertility and Development, 2010, 22(Supplement)

A Novel Large-Scale Deletion of The Mitochondrial DNA of Spermatozoa of Men in North Iran